CST, 53988SF, SLAMF6/CD352 (E3H1E) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying SLAMF6. Validated for Western Blotting,Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, SLAMF6/CD352 (E3H1E) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #53988) is ready to ship. Product Usage Information This product is the carrier-free version of product #60859. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN, or CUT&Tag assays. If you require a carrier-free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 60859 . Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity SLAMF6/CD352 (E3H1E) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total SLAMF6/CD352 protein. This antibody does not cross-react with SLAMF3/Ly9, SLAMF4/2B4, or SLAMF5/CD84 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human SLAMF6/CD352 protein. Background SLAMF6 (CD352/NTB-A) is a type-I transmembrane glycoprotein belonging to the signaling lymphocytic activation molecule (SLAM) family of immunomodulatory receptors. Like other members of the SLAM receptor family, SLAMF6 contains Ig-like domains within its extracellular region and conserved tyrosine-based signaling motifs within its intracellular domain that, when phosphorylated, bind to the SAP and EAT-2 signaling adaptors (1). SLAMF6 is expressed on the surface of multiple types of immune cells, such as those of the B, T, and NK lineages. Its activation is triggered by homotypic interactions involving its extracellular domain (1-3). Indeed, research studies have shown that in T cells, SLAMF6 engagement facilitates activation and cytokine production (4). Similarly, homotypic ligand-mediated engagement of SLAMF6 on NK cells activates signaling cascades that drive proliferation, cytotoxicity, and cytokine production (1,5-7). Alternate Names Activating NK receptor; CD352; KALI; KALIb; Ly108; MGC104953; natural killer-, T- and B-cell antigen; NK-T-B-antigen; NTB-A; NTBA; NTBA receptor; SF2000; SLAF6; SLAM family member 6; SLAMF6 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 60 Source/Isotype: Rabbit IgG