CST, 54043T, Annexin V (E3W8V) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying ANXA5. Validated for WB,IHC,IF,F. Available in 2 sizes. Highly specific and rigorously validated in-house, Annexin V (E3W8V) Rabbit Monoclonal Antibody (CST #54043) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:50 - 1:200 Immunofluorescence (Immunocytochemistry): 1:100 - 1:400 Flow Cytometry (Fixed/Permeabilized): 1:100 - 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 24122 . Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity Annexin V (E3W8V) Rabbit Monoclonal Antibody recognizes endogenous levels of total annexin V protein. Species reactivity for IF-IC is human only. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Thr10 of human annexin V protein. Background Annexin V, also known as PAP-1 or Lipocortin V, is an ~30 kDa protein that binds to phospholipids in a calcium-dependent manner (1). All annexins contain a putative PKC binding site, but only annexin V has been identified as an inhibitor of this pathway (2). Via direct interaction with VEGFR-2 receptor, annexin V may also signal to regulate vascular endothelial cell proliferation (3). Annexin V preferentially binds phosphatidylserine in competition with prothrombin, leading to inhibition of blood coagulation at sites of injury preceding contact between lipid components and coagulation factors that initiate thrombosis (4-6). The ability of annexin V to bind specifically and robustly to phosphatidylserine makes it an attractive reagent in detecting apoptotic cells (7). Annexin V is inducible by glucocorticoids and can be phosphorylated by tyrosine and serine/threonine kinases (8). It is thought to block the production of inflammation mediators, such as prostaglandins and leukotrienes by inhibiting the release of arachidonic acid from membranes by phospholipase A2 (8). Alternate Names Anchorin CII; Annexin A5; Annexin V; Annexin-5; ANX5; anxa 5; ANXA5; Calphobindin I; CBP-I; Endonexin II; ENX2; epididymis secretory protein Li 7; HEL-S-7; Lipocortin V; PAP-I; Placental anticoagulant protein 4; Placental anticoagulant protein I; PP4; RPRGL3; Thromboplastin inhibitor; VAC-alpha; Vascular anticoagulant-alpha Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 30 Source/Isotype: Rabbit IgG