CST, 54081SF, Lysozyme C (E9W6E) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying LYZ1. Validated for Western Blotting,Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, Lysozyme C (E9W6E) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #54081) is ready to ship. Product Usage Information This product is the carrier free version of product #67459. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 67459 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Lysozyme C (E9W6E) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total Lysozyme C protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to full-length human Lysozyme C protein. Background Lysozymes are secreted proteins that have bacteriolytic function which are critical for mammalian innate immune function. All lysozymes function to defend host animals from microbial infection by the hydrolysis of bacterial cell wall peptidoglycan (1). Conventional-type lysozymes (Lysozyme C) are one of three types of lysozymes; each family member is categorized based on amino acid sequence and biochemical properties. Lysozyme C is expressed in mammalian secretions like tears, urine, and milk but is also expressed by phagocytes such as macrophages, neutrophils, and dendritic cells. Lysozyme C is encoded in humans by a single gene. The mouse orthologs of Lysozyme C are encoded by two genes, and , which encode Lysozyme C-1 and Lysozyme C-2 (Lysozyme C-1/2). Interestingly, is upregulated in microglia of Alzheimer's disease mouse model brains that have been stimulated by specific forms of activity (2). and are uniquely expressed in microglia, and increased correlates with microglia-mediated β-amyloid (Aβ) clearance, suggesting that Lysozyme C-1/2 may directly contribute to microglial-clearance of Aβ or act as a marker for certain microglial activity states in the brain (3). Alternate Names 1,4-beta-N-acetylmuramidase C; c-type lysozyme; LYSC; lysozyme; lysozyme (renal amyloidosis); Lysozyme C; lysozyme F1; LYZ; LYZF1; LZM Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 15 Source/Isotype: Rabbit IgG