CST, 54113T, NUSAP1 (F1H1E) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying NUSAP1. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, NUSAP1 (F1H1E) Rabbit Monoclonal Antibody (CST #54113) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity NUSAP1 (F1H1E) Rabbit Monoclonal Antibody recognizes endogenous levels of total NUSAP1 protein. Based on the sequence of the peptide antigen, this antibody is expected to detect all isoforms of NUSAP1. This antibody detects approximately 35 kDa and 45 kDa proteins of unknown identity in some cell lines. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys155 of human NUSAP1 protein. Background The nucleolar and spindle-associated protein 1 (NUSAP1) is a microtubule-associated protein and mitotic regulator of spindle assembly, chromosome segregation, and cytokinesis. NUSAP1 is often highly expressed in human cancer, and its expression is associated with tumor progression and poor prognosis in various cancer types (1-4). Alternate Names ANKT; BM037; FLJ13421; LNP; nucleolar and spindle associated protein 1; Nucleolar and spindle-associated protein 1; nucleolar protein ANKT; NuSAP; NUSAP1; PRO0310p1; Q0310; SAPL Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 55-60 Source/Isotype: Rabbit IgG