CST, 54141S, AMBRA1 (F4O7X) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying AMBRA1. Validated for Western Blotting,Simple Western™,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, AMBRA1 (F4O7X) Rabbit Monoclonal Antibody (CST #54141) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:250 Immunoprecipitation: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity AMBRA1 (F4O7X) Rabbit Monoclonal Antibody recognizes endogenous levels of total AMBRA1 protein. This antibody detects a 40 kDa protein of unknown identity in some cell lines. Species Reactivity: Human, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to human AMBRA1 protein. Background Activating molecule in Beclin1-regulated autophagy (AMBRA1) is a WD40-containing protein expressed during neurodevelopment that is required for neural tube development and autophagy (1). Several studies have identified interactions between AMBRA1 and regulators of autophagy and apoptosis (reviewed in 2). AMBRA1 was originally found to interact with Beclin-1, a key protein responsible for activating the class III PI3K Vps34 (1). Further studies showed that AMBRA1 tethers the Beclin-1-Vps34 complex to the cytoskeletal network through dynein light chains and that during autophagy, ULK1 phosphorylates AMBRA1, resulting in disassociation with dynein and translocation of the Beclin-Vps34 complex to the endoplasmic reticulum to initiate autophagosome formation (3,4). In addition, it has been found that AMBRA1 binds to mitochondrial Bcl-2 and that this interaction is regulated by either apoptosis or autophagy (5,6). AMBRA1 also interacts with Parkin, an E3 ubiquitin ligase important for mitophagy, a selective autophagic process of mitochondrial clearance (7,8). Alternate Names activating molecule in beclin-1-regulated autophagy; Activating molecule in BECN1-regulated autophagy protein 1; AMBRA1; AMRA1; autophagy and beclin 1 regulator 1; autophagy/beclin-1 regulator 1; DCAF3; DDB1 and CUL4 associated factor 3; FLJ20294; KIAA1736; MGC33725; WD repeat domain 94; WDR94 Specification REACTIVITY: H R SENSITIVITY: Endogenous MW (kDa): 135-150 Source/Isotype: Rabbit IgG