CST, 54155S, OAS2 Antibody

Polyclonal Antibody for studying OAS2. Validated for Western Blotting. Highly specific and rigorously validated in-house, OAS2 Antibody (CST #54155) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity OAS2 Antibody recognizes endogenous levels of total OAS2 protein. This antibody cross-reacts with an unidentified protein of 29 kDa in some cell lines. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala330 of human OAS2 protein. Antibodies are purified by peptide affinity chromatography. Background 2'-5'-oligoadenylate synthetase 2 (OAS2) is an enzyme induced by type 1 interferon that plays a key role in the host antiviral innate immune response (1,2). The OAS family of proteins includes OAS1, OAS2, OAS3, and OASL in humans (3). OAS2 is activated by cytosolic dsRNA and its enzymatic activity generates the second messenger 2'-5'-linked oligoadenylate. These 2'-5'-linked oligoadenylates bind to the ribonuclease RNase L, which then degrades viral and cellular RNA in an effort to curb viral replication (1). Research studies have shown that components of the OAS-RNase L system, including OAS2, are highly upregulated in response to SARS-CoV-2 infection, suggesting a role for this antiviral pathway in the pathogenesis of SARS-CoV-2 (4,5). Alternate Names (2-5')oligo(A) synthase 2; (2-5')oligo(A) synthetase 2; (2'-5')oligo(A) synthetase 2; 2-5A synthase 2; 2-5A synthetase 2; 2'-5'-oligoadenylate synthase 2; 2'-5'-oligoadenylate synthetase 2; 2'-5'-oligoadenylate synthetase 2, 69/71kDa; 2'5' oligoadenylate synthetase 2; MGC78578; OAS2; p69 OAS / p71 OAS; p69OAS / p71OAS Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 70 SOURCE: Rabbit