CST, 5417S, GCNF Antibody

Polyclonal Antibody for studying NR6A1. Validated for Western Blotting. Highly specific and rigorously validated in-house, GCNF Antibody (CST #5417) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity GCNF Antibody detects endogenous levels of total GCNF protein. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic polypeptide corresponding to residues surrounding Ala349 of human GCNF. Antibodies are purified by Protein A and peptide affinity chromatography. Background GCNF (Germ Cell Nuclear Factor), also known as NR6A1 (Nuclear Receptor Subfamily 6 Group A member), is an orphan member of the nuclear receptor gene superfamily (1). It has been shown to be expressed in the nervous system during development and during specific stages in maturing germ cells of the ovary and testis in the adult, and has probable roles in gametogenesis, neurogenesis, and normal embryonic development during gastrulation (1,2). Inactivation of GCNF in mouse results in abnormal posterior development, impaired midbrain development, insufficient closure of the neural tube, and eventual embryonic death (3). GCNF has been shown to be a repressor of OCT-4 and of the protamine genes (4,5) and plays a critical role in the control of gene expression during embryogenesis and spermatogenesis (2,6). Alternate Names CT150; GCNF; GCNF1; Germ cell nuclear factor; germ cell nuclear factor variant 1; hGCNF; hRTR; NR61; NR6A1; Nuclear receptor subfamily 6 group A member 1; nuclear receptor subfamily 6, group A, member 1; retinoic acid receptor-related testis-associated receptor; Retinoid receptor-related testis-specific receptor; RTR Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 50 SOURCE: Rabbit