CST, 54192T, CD15/SSEA1 (MMA) Mouse Monoclonal Antibody

Monoclonal Antibody for studying FUT4. Validated for IHC Leica Bond,Immunohistochemistry (Paraffin),Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, CD15/SSEA1 (MMA) Mouse Monoclonal Antibody (CST #54192) is ready to ship. Product Usage Information IHC Leica Bond: 1:50 - 1:200 Immunohistochemistry (Paraffin): 1:50 - 1:200 Immunofluorescence (Immunocytochemistry): 1:50 - 1:200 Storage Supplied as liquid tissue culture supernatant containing sodium azide as a preservative. Stable for 6 months when stored at 4°C. Do not aliquot the antibody. Note: This product is only available in the format listed above. We are unable to provide this in a carrier free, custom formulation. Protocol Available protocols: IHC Leica Bond, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity CD15/SSEA1 (MMA) Mouse Monoclonal Antibody recognizes endogenous levels of CD15/SSEA1 protein. Species Reactivity: Human, Mouse, Monkey Source / Purification Monoclonal antibody is produced by immunizing BALB/C mice injected with U-937 histiocytic cell line. Background SSEA1 antibody detects a lactoseries oligosaccharide antigen that is expressed on the surface of mouse embryonal carcinoma and embryonic stem cells (1). This antigen is also found on early mouse embryos and both mouse and human germ cells, but is absent on human embryonic stem and carcinoma cells. Expression of SSEA1 in these human cell types increases upon differentiation, while on the mouse cell types differentiation leads to decreased expression (2). Alternate Names alpha (1,3) fucosyltransferase, myeloid-specific; Alpha-(1,3)-fucosyltransferase; Alpha-(1,3)-fucosyltransferase 4; CD15; ELAM ligand fucosyltransferase; ELAM-1 ligand fucosyltransferase; ELFT; FCT3A; Fuc-TIV; Fucosyltransferase 4; fucosyltransferase 4 (alpha (1,3) fucosyltransferase, myeloid-specific); Fucosyltransferase IV; FucT-IV; FUT4; FUTIV; Galactoside 3-L-fucosyltransferase; Lewis X; LeX; SSEA-1; stage-specific embryonic antigen 1 Specification REACTIVITY: H M Mk SENSITIVITY: Endogenous Source/Isotype: Mouse IgM