CST, 5419S, ZFX (L28B6) Mouse Monoclonal Antibody

Monoclonal Antibody for studying ZFX. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, ZFX (L28B6) Mouse Monoclonal Antibody (CST #5419) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity ZFX (L28B6) Mouse Monoclonal Antibody detects endogenous levels of total ZFX protein. This antibody may cross-react with ZFY protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a recombinant fragment of human ZFX. Background The ZFX (X-linked zinc finger protein) gene is expressed from the inactive X chromosome and is structurally similar to its homologue on the Y chromosome, ZFY (1). Transcripts of ZFX and ZFY encode proteins composed of a highly acidic amino-terminal domain and a carboxy-terminal zinc-finger motif that is commonly associated with nucleic acid-binding proteins (2). Both ZFY and ZFX are probable transcriptional activators and may function in sex determination (3). Multiple alternatively spliced transcript variants of ZFX, encoding different isoforms, have been identified and may be functionally distinct (2). Conditional gene targeting studies in mouse have suggested ZFX is also required for self-renewal of embryonic and hematopoietic stem cells (4). ZFX has also been suggested to play a role in proliferation and expansion of B cells, and could contribute to lymphocyte homeostasis (5). Alternate Names X-linked zinc finger protein; ZFX; zinc finger protein X-linked; zinc finger protein, X-linked; Zinc finger X-chromosomal protein; ZNF926 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 135 Source/Isotype: Mouse IgG2b