CST, 5421S, Bub1b (D32E8) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Bub1B. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Bub1b (D32E8) Rabbit Monoclonal Antibody (CST #5421) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Bub1b (D32E8) Rabbit Monoclonal Antibody detects endogenous levels of total Bub1b protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to amino acid sequence surrounding Lys370 of human Bub1b. Background The Mitotic Checkpoint Complex (MCC), which contains Bub1, Bub1b, Bub3, Mad2, and Cdc20, controls chromosome segregation and monitors kinetochore-microtubule interactions (1). During mitosis, the MCC complex inhibits the ubiquitin ligase activity of the Anaphase Promoting Complex/Cyclosome (APC/C), thereby preventing cells with unaligned chromosomes from prematurely entering anaphase (2). Research studies have shown that Bub1b and Bub1 kinases are mutated in several types of human malignancies including hematopoietic, colorectal, lung, and breast cancers (3). Biallelic mutations in Bub1b have been found in mosaic variegated aneuploidy syndrome and premature chromatid separation syndrome (4). Bub1b mouse germline knockouts are embryonic lethal with heterozygous animals displaying genetic instability, early aging phenotypes, and increased cancer susceptibility (5). Bub3 binds both Bub1 and Bub1b, facilitating their recruitment to kinetochores (6), and is required for functional microtubule-kinetochore interactions (7). Alternate Names BUB1 mitotic checkpoint serine/threonine kinase B; Bub1A; BUB1B; BUB1B, mitotic checkpoint serine/threonine kinase; BUB1beta; BUBR1; budding uninhibited by benzimidazoles 1 homolog beta; budding uninhibited by benzimidazoles 1 homolog beta (yeast); hBUBR1; MAD3/BUB1-related protein kinase; MAD3L; Mitotic checkpoint kinase MAD3L; Mitotic checkpoint serine/threonine-protein kinase BUB1 beta; MVA1; Protein SSK1; SSK1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 130 Source/Isotype: Rabbit