CST, 54232S, MAP1S Antibody

Polyclonal Antibody for studying BPY2-IP1 mouse. Validated for Western Blotting. Highly specific and rigorously validated in-house, MAP1S Antibody (CST #54232) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity MAP1S Antibody recognizes endogenous levels of total MAP1S protein. Species Reactivity: Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg499 of mouse MAP1S protein. Antibodies are purified by peptide affinity chromatography. Background MAP1S is a member of the microtubule-associated protein (MAP) family. There are at least ten proteins in the MAP family, including MAP1A, MAP1B, MAP1S, MAP2, MAP6, MAP7, MAP9, Tau, DCLK, and DCX. MAP1 subfamily members express predominantly in brain cells regulating several physiological processes in cells, including cytoskeleton regulation that, in some cases are energy-dependent, including the autophosphorylation and phosphorylation of other MAP family members, and other cytoskeletal proteins (1,2). MAP1 family members have been identified as high-molecular-weight proteins consisting of the full-length (FL), at least one heavy chain (HC), one light chain (LC), and, in some members short chains (SC) (2,3). The FL-MAP1S is formed by the HC and LC, forming the active MAP1S complex. The LC binds, bundles, and stabilizes microtubules, and the HC regulates LC activity (2). MAP1S also interacts with Nemitin (neuronal enriched MAP interacting protein), enhancing cytoskeleton regulation (4). MAP1S contributes to mitochondria stabilization by interacting with mitochondrion-associated leucine-rich PPR-motif containing protein (LRPPRC). MAP1S regulates ubiquitination processes like autophagosome biogenesis and protein clearance. These processes are MAP1S isoform-specific (5). An increased level of MAP1S enhances autophagy to remove p62-associated aggresomes and dysfunctional organelles that trigger DNA double-strand breaks and genome instability (6). Alternate Names 6430517J16Rik; Bpy2; BPY2 interacting protein 1; BPY2-interacting protein 1; BPY2-IP1; Bpy2ip1; MAP-1S; Map1s; MAP1S heavy chain; MAP1S light chain; Map8; Microtubule-associated protein 1S; Microtubule-associated protein 8; Mta; Mtap1s; VCY2; VCY2IP1 Specification REACTIVITY: M R SENSITIVITY: Endogenous MW (kDa): 125, 142 SOURCE: Rabbit