CST, 54262S, UCP1 (E9Z2V) Rabbit Monoclonal Antibody (Alexa Fluor® 488 Conjugate)

Monoclonal Antibody for studying UCP1. Validated for Immunofluorescence (Frozen). Highly specific and rigorously validated in-house, UCP1 (E9Z2V) Rabbit Monoclonal Antibody (Alexa Fluor^® 488 Conjugate) (CST #54262) is ready to ship. Product Usage Information Immunofluorescence (Frozen): 1:50 - 1:200 Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Immunofluorescence (Frozen) Specificity / Sensitivity UCP1 (E9Z2V) Rabbit Monoclonal Antibody (Alexa Fluor Species Reactivity: Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro152 of human UCP1 protein. Background Uncoupling protein 1 (UCP1) is a mitochondrial inner membrane transport protein that is primarily expressed in brown adipose tissue (BAT). UCP1 dissipates the pH gradient resulting from oxidative phosphorylation, which uncouples ATP synthesis from oxidative phosphorylation and leads to the release of heat energy. As a result, UCP1 plays an important role in thermogenesis (reviewed in 1). Research studies indicate that subcutaneous white adipose depots in mice contain beige adipocytes that express low levels of UCP1 protein (2). Additional studies show possible differences in thermogenesis in individuals carrying specific polymorphisms in the corresponding gene (3). Related studies link UCP1 to the possible development of obesity and type 2 diabetes (4). Alternate Names Mitochondrial brown fat uncoupling protein 1; SLC25A7; Solute carrier family 25 member 7; Thermogenin; UCP; UCP 1; UCP1; uncoupling protein 1; uncoupling protein 1 (mitochondrial, proton carrier) Specification REACTIVITY: M R SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG