CST, 5426S, Phospho-Talin (Ser425) Antibody

Polyclonal Antibody for studying Talin1 (Ser425) phosphate. Validated for Western Blotting. Highly specific and rigorously validated in-house, Phospho-Talin (Ser425) Antibody (CST #5426) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Phospho-Talin (Ser425) Antibody detects endogenous levels of talin protein only when phosphorylated at Ser425. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser425 of human talin protein. Antibodies are purified using protein A and peptide affinity chromatography. Background Focal adhesions connect the cytoskeleton with the extracellular matrix (ECM), a complex structure of secreted macromolecules that surrounds mammalian organs and tissues. Integrins clustered on the extracellular side of focal adhesions signal from the ECM to intracellular protein complexes, which in turn signal to the actin cytoskeleton to regulate the tension needed for cell motility. Internal signals also converge on focal adhesions to regulate integrin affinity and avidity. Signaling through focal adhesions regulates cell adhesion, migration, proliferation, apoptosis, and gene expression, and impacts cellular processes such as development, wound healing, immune response, invasion, metastasis, and angiogenesis (reviewed in 1-3). Talin is a large, multidomain focal adhesion protein that interacts with the intracellular domains of integrins and other focal adhesion proteins. Talin is involved in the formation of focal adhesions and in linking focal adhesions to the actin cytoskeleton (4). The interaction between talin and integrins increases the affinity between integrin and both insoluble and soluble ECM proteins (5,6). Phosphorylation of talin at Ser425 was discovered using mass spectrometry. This site is a potential substrate of CDK5 (7). Alternate Names ILWEQ; KIAA1027; talin 1; Talin-1; talin1; TLN; TLN1 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 270 SOURCE: Rabbit