CST, 5432T, RACK1 (D59D5) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Rack1. Validated for WB,IP,IHC,eCLIP. Available in 2 sizes. Highly specific and rigorously validated in-house, RACK1 (D59D5) Rabbit Monoclonal Antibody (CST #5432) is ready to ship. Product Usage Information For more information about the RBP-eCLIP service please visit Eclipsebio . Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunohistochemistry (Paraffin): 1:400 - 1:1600 eCLIP: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 37124 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin) Specificity / Sensitivity RACK1 (D59D5) Rabbit Monoclonal Antibody recognizes endogenous levels of total RACK1 protein. Species Reactivity: Human, Mouse, Rat, Monkey, D. melanogaster Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to central residues of human RACK1 protein. Background The highly conserved receptor for activated C kinase 1 (RACK1), homologous to the β subunit of heterotrimeric G-proteins, was originally identified through its binding of active PKCβII and other classical PKC isoforms (1). RACK1 is a scaffold protein that recruits PKC and a wide range of other proteins to specific subcellular locations, promoting the formation of multiprotein complexes to induce and integrate various signaling pathways (reviewed in 2). One example of this is its enhancement of PKC-dependent JNK activation (3). RACK1 protein also resides in the eukaryotic ribosome, suggesting the possibility that RACK1 participates in the assembly of signaling complexes that regulate translation as well (reviewed in 4). RACK1 binds the SH2 domain of Src, and phosphorylation of RACK1 by Src occurs at Tyr228 after PKC activation (5). Alternate Names Cell proliferation-inducing gene 21 protein; GBLP; Gnb2-rs1; GNB2L1; guanine nucleotide binding protein (G protein), beta polypeptide 2-like 1; guanine nucleotide binding protein beta polypeptide 2-like 1; Guanine nucleotide-binding protein subunit beta-2-like 1; Guanine nucleotide-binding protein subunit beta-2-like 1, N-terminally processed; Guanine nucleotide-binding protein subunit beta-like protein 12.3; H12.3; HLC-7; Human lung cancer oncogene 7 protein; lung cancer oncogene 7; PIG21; proliferation-inducing gene 21; protein homologous to chicken B complex protein, guanine nucleotide binding; RACK1; Receptor for activated C kinase; Receptor for Activated C Kinase 1; Receptor of activated protein C kinase 1; Receptor of activated protein C kinase 1, N-terminally processed; Receptor of activated protein kinase C 1; Small ribosomal subunit protein RACK1 Specification REACTIVITY: H M R Mk Dm SENSITIVITY: Endogenous MW (kDa): 32 Source/Isotype: Rabbit IgG