CST, 54364T, Phospho-LAT (Tyr255) (E3K6B) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying LAT (Tyr255) phosphate. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-LAT (Tyr255) (E3K6B) Rabbit Monoclonal Antibody (CST #54364) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Phospho-LAT (Tyr255) (E3K6B) Rabbit Monoclonal Antibody recognizes endogenous levels of LAT protein only when phosphorylated at Tyr255. This antibody cross-reacts with a 70 kDa phospho-protein of unknown origin in some treated cell lines. Tyr255 of LAT, long isoform corresponds to Tyr226 of LAT, short isoform. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr255 of human LAT protein, isoform 1. Background LAT, a transmembrane adaptor protein expressed in T, NK, and mast cells, is an important mediator for T cell receptor (TCR) signaling (1). Upon TCR engagement, activated Zap-70 phosphorylates LAT at multiple conserved tyrosine residues within SH2 binding motifs, exposing these motifs as the docking sites for downstream signaling targets (2,3). The phosphorylation of LAT at Tyr171 and Tyr220 enables the binding of Grb2, Gads/SLP-76, PLCγ1, and PI3 kinase through their SH2 domain and translocates them to the membrane. This process eventually leads to activation of the corresponding signaling pathways (1-4). Research studies have shown that LAT is also phosphorylated at Tyr225 by Zap-70 and Syk following activation of the TCR, which facilitates its binding to Grb2 and Vav and subsequent downstream activation of ERK (3-5). Alternate Names 36 kDa phospho-tyrosine adapter protein; 36 kDa phospho-tyrosine adaptor protein; IMD52; LAT; LAT1; linker for activation of T cells; linker for activation of T cells, transmembrane adaptor; Linker for activation of T-cells family member 1; p36-38; pp36 Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 40 Source/Isotype: Rabbit IgG