CST, 54376T, MMP-1 (E9S9N) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying MMP1, active form. Validated for WB,IP,IHC,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, MMP-1 (E9S9N) Rabbit Monoclonal Antibody (CST #54376) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Immunohistochemistry (Paraffin): 1:100 - 1:400 Immunofluorescence (Immunocytochemistry): 1:400 - 1:1600 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 87303 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity MMP-1 (E9S9N) Rabbit Monoclonal Antibody recognizes endogenous levels of total MMP-1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val267 of human MMP-1 protein. Background The matrix metalloproteinase (MMP) family of proteases is a group of zinc-dependent enzymes that target extracellular proteins, including growth factors, cell surface receptors, adhesion molecules, matrix structural proteins, and other proteases (1,2). Within this family, MMP-1, MMP-8, and MMP-13 have been characterized as a collagenase subfamily of MMPs targeting fibrillar collagen (collagen type I, II, and III) for degradation. In addition to collagen, MMP-1 also has activity toward a broad array of other ECM proteins, such as fibronectin, gelatin, aggrecan (etc.), as well as growth factors, chemokines, and cytokines (3). MMP-1 is widely involved in tissue remodeling during wound healing, tumor growth, invasion and metastasis, and arthritis (4-6). Alternate Names 22 kDa interstitial collagenase; 27 kDa interstitial collagenase; CLG; CLGN; Collagenase; Fibroblast collagenase; Interstitial collagenase; matrix metallopeptidase 1; matrix metallopeptidase 1 (interstitial collagenase); matrix metalloprotease 1; Matrix metalloproteinase-1; MMP-1; MMP1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 55 Source/Isotype: Rabbit IgG