CST, 54382SF, Phospho-TBK1/NAK (Ser172) (D52C2) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying TBK1 (Ser172) phosphate. Validated for WB,IF,F,ELISA+. Highly specific and rigorously validated in-house, Phospho-TBK1/NAK (Ser172) (D52C2) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #54382) is ready to ship. Product Usage Information This product is the carrier free version of product #5483. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 5483 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Phospho-TBK1/NAK (Ser172) (D52C2) Rabbit Monoclonal Antibody (BSA and Azide Free) detects endogenous levels of TBK1 only when phosphorylated at Ser172. This antibody may cross-react with phospho-IKKε. This antibody lacks sensitivity in fixed frozen mouse tissue by indirect immunofluorescent detection. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is prepared from animals immunized with a synthetic phosphopeptide corresponding to residues surrounding Ser172 of human TBK1. Background TBK1 (TANK-binding kinase 1)/NAK (NF-κB activating kinase) is an IκB kinase (IKK)-activating kinase and can activate IKK through direct phosphorylation (1). TBK1 was identified through association with the TRAF binding protein, TANK, and found to function upstream of NIK and IKK in the activation of NF-κB (2). TBK1 induces IκB degradation and NF-κB activity through IKKβ. TBK1 may mediate IKK and NF-κB activation in response to growth factors that stimulate PKCε activity (1). TBK1 plays a pivotal role in the activation of IRF3 in the innate immune response (3). TBK1 is phosphorylated at Ser172 within its activation loop, which is necessary of its kinase activity including the downstream phosphorylation of IRF3 (4,5). Alternate Names FLJ11330; FTDALS4; IIAE8; NAK; NF-kappa-B-activating kinase; NF-kB-activating kinase; Serine/threonine-protein kinase TBK1; T2K; TANK binding kinase 1; TANK-binding kinase 1; TBK1 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 84 Source/Isotype: Rabbit IgG