CST, 5439T, SKAR alpha/beta (D65E8) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SKAR. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, SKAR alpha/beta (D65E8) Rabbit Monoclonal Antibody (CST #5439) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Immunocytochemistry): 1:3200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity SKAR alpha/beta (D65E8) Rabbit Monoclonal Antibody detects endogenous levels of total SKAR protein, α and β isoforms. By western blot, this antibody detects an approximately 21 kDa band of unknown origin in all primate cell types tested. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of human SKAR. Background p70 S6 kinase is a mitogen activated Ser/Thr protein kinase downstream of phosphoinositide-3 kinase (PI3K) and the target of rapamycin, FRAP/mTOR. p70 S6 kinase is required for cell growth and cell cycle progression (1,2). SKAR is a recently discovered substrate of S6K1. SKAR exists in two isoforms, α and β, the latter having a 29 amino acid truncation. Phosphorylation of SKAR is mitogen-induced and sensitive to rapamycin. Reduction in SKAR protein levels results in decreased cell size, further implicating SKAR in cell growth control (3). Alternate Names 46 kDa DNA polymerase delta interaction protein; DNA polymerase delta interacting protein 3; KIAA1649; p46; PDIP3; PDIP46; POLDIP3; polymerase (DNA-directed), delta interacting protein 3; polymerase (DNA) delta interacting protein 3; polymerase delta interacting protein 46; Polymerase delta-interacting protein 3; RNA-binding protein P46; S6K1 Aly/REF-like target; SKAR Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 46, 43 Source/Isotype: Rabbit IgG