CST, 5440S, LRP5 (D5G4) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying LRP5. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, LRP5 (D5G4) Rabbit Monoclonal Antibody (CST #5440) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity LRP5 (D5G4) Rabbit Monoclonal Antibody detects endogenous levels of total LRP5 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a recombinant human LRP5 protein (extracellular region). Background LRP5 and LRP6 are single-pass transmembrane proteins belonging to the low-density lipoprotein receptor (LDLR)-related protein family. Unlike other members of the LDLR family, LRP5 and LRP6 have four EGF and three LDLR repeats in the extracellular domain, and proline-rich motifs in the cytoplasmic domain (1). They function as co-receptors for Wnt and are required for the canonical Wnt/β-catenin signaling pathway (2,3). LRP5 and LRP6 are highly homologous and have redundant roles during development (4,5). The activity of LRP5 and LRP6 can be inhibited by the binding of some members of the Dickkopf (DKK) family of proteins (6,7). Upon stimulation with Wnt, LRP6 is phosphorylated at multiple sites including Thr1479, Ser1490, and Thr1493 by kinases such as GSK-3 and CK1 (8-10). Phosphorylated LRP6 recruits axin to the membrane and presumably activates β-catenin signaling (8-10). LRP5 is involved in the regulation of bone homeostasis. Mutations and polymorphisms in LPR5 are associated with bone diseases like osteoporosis-pseudoglioma syndrome and high-bone-mass disorders (11-13). In addition, mutations in LRP5 are found in patients with hyperparathyroid tumor and breast cancer (14,15). Alternate Names BMND1; EVR1; EVR4; HBM; LDL receptor related protein 5; low density lipoprotein receptor-related protein 5; low density lipoprotein receptor-related protein 7; Low-density lipoprotein receptor-related protein 5; Low-density lipoprotein receptor-related protein 7; LR3; LRP-5; LRP-7; LRP5; LRP7; OPPG; OPS; OPTA1; PCLD4; VBCH2 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 200 Source/Isotype: Rabbit IgG