CST, 54488S, MEKK2 (E6C2G) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying MEKK2. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, MEKK2 (E6C2G) Rabbit Monoclonal Antibody (CST #54488) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity MEKK2 (E6C2G) Rabbit Monoclonal Antibody recognizes endogenous levels of total MEKK2 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His244 of human MEKK2 protein. Background Mitogen-activated protein kinase kinase kinase 2 (MEKK2/MAP3K2) belongs to the MAP3K family of Ser/Thr kinases. Research studies have demonstrated that MEKK2 plays a pivotal role in transducing mitogenic signals emanating from EGFR and FGF2R to JNK and ERK5 signaling cascades (1,2). Post-translationally, MEKK2 is regulated through multiple mechanisms, including dimerization (3,4), ubiquitination (5,6), phosphorylation (7), and methylation (8). Research studies implicate dysregulation of MEKK2 signaling in breast carcinoma (9), colorectal carcinoma (10), and pancreatic ductal adenocarcinoma (8). Alternate Names M3K2; MAP/ERK kinase kinase 2; MAP3K2; MAPK/ERK kinase kinase 2; MAPKKK2; MEK kinase 2; MEKK 2; MEKK2; MEKK2B; Mitogen-activated protein kinase kinase kinase 2 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 78 Source/Isotype: Rabbit IgG