CST, 5449T, Thymidylate Synthase (D26G11) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Thymidylate Synthase. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Thymidylate Synthase (D26G11) Rabbit Monoclonal Antibody (CST #5449) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Thymidylate Synthase (D26G11) Rabbit Monoclonal Antibody detects endogenous levels of total Thymidylate Synthase protein. Species Reactivity: Human, Mouse, Rat, Hamster, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to central residues of human Thymidylate Synthase protein. Background The methylation of deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP) is an essential step in the formation of thymine nucleotides (1,2, reviewed in 3). This process is catalyzed by thymidylate synthase (TS or TYMS), a homodimer composed of two 30 kDa subunits. TS is an intracellular enzyme that provides the sole source of thymidylate, making it a required enzyme in DNA biosynthesis with activity highest in proliferating cells (1). Being the exclusive source of dTMP, investigators have concluded that TS is also an important target for anticancer agents such as 5-fluorouracil (5-FU) (1-5). 5-FU acts as a TS inhibitor and is active against solid tumors such as colon, breast, head, and neck. Research studies have demonstrated that patients with metastases expressing lower levels of TS have a higher response rate to treatment with 5-FU than patients with tumors that have increased levels of TS (5). Researchers continue to investigate TS expression in different types of cancers (6-10). Alternate Names HST422; MGC88736; Thymidylate synthase; thymidylate synthetase; TMS; TS; TSase; TYMS; TYSY Specification REACTIVITY: H M R Hm Mk SENSITIVITY: Endogenous MW (kDa): 30 Source/Isotype: Rabbit IgG