CST, 5451S, PEN2 Antibody

Polyclonal Antibody for studying PEN2. Validated for Western Blotting. Highly specific and rigorously validated in-house, PEN2 Antibody (CST #5451) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity PEN2 Antibody detects endogenous levels of total PEN2 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys11 of human PEN2 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Presenilin Enhancer 2 (PEN2) is a small integral membrane glycoprotein that contains two recognized transmembrane domains. Both the N- and C-terminal domains are oriented into the lumen of the endoplasmic reticulum (1). PEN2, along with Presenilin 1, Presenilin 2, Nicastrin, and APH-1 form the protein complex γ-secretase (2). The proteinase BACE catalyses the initial step in APP processing by cleaving and releasing soluble APPβ (3). The remaining membrane bound APP is then cleaved by the γ-secretase complex, causing the release of amyloid β-peptide, the main constituent of amyloid plaques. These plaques are a hallmark of Alzheimer's disease pathology (2). In addition to APP, the γ-secretase complex cleaves several other proteins and necessary presenilin-dependent signaling cascades, including the Notch pathway (4). It was found that PEN2 is an important part of the γ-secretase complex, and knocking it down results in reduced amounts of the complex, resulting in a loss of γ-secretase activity (5). Alternate Names ACNINV2; Gamma-secretase subunit PEN-2; hematopoietic stem/progenitor cells protein MDS033; MDS033; MSTP064; PEN-2; PEN2; presenilin enhancer 2 homolog; presenilin enhancer 2 homolog (C. elegans); Presenilin enhancer protein 2; presenilin enhancer, gamma-secretase subunit; PSENEN Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 13 SOURCE: Rabbit