CST, 54542S, Phospho-HNF1 alpha (Ser247) Antibody

Polyclonal Antibody for studying HNF1alpha (Ser247) phosphate. Validated for Western Blotting. Highly specific and rigorously validated in-house, Phospho-HNF1 alpha (Ser247) Antibody (CST #54542) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Phospho-HNF1 alpha (Ser247) Antibody recognizes endogenous levels of HNF1α protein only when phosphorylated at Ser247. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser247 of human HNF1α protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Hepatocyte nuclear factor 1α (HNF1α, also known as TCF1 or MODY3) is a transcription factor that plays a role in the tissue-specific regulation of liver gene expression (1). Research has shown that heterogeneous mutations of HNF1α are linked to maturity onset diabetes of the young (MODY) (2). Recent studies indicate that increased concentrations of free fatty acids can reduce the expression of FoxA2/HNF3β and HNF1α in pancreatic β-cells and lead to their nuclear exclusion, resulting in symptoms of several metabolic syndromes (3). HNF1α is inhibited through Akt2-mediated phosphorylation at Ser247 and is a negative regulator of PPARγ gene transcription. Research studies have shown that inhibition of PPARγ is beneficial in steatosis-associated liver cancer in mouse models (4). Alternate Names albumin proximal factor; hepatic nuclear factor 1; Hepatocyte nuclear factor 1-alpha; HNF-1-alpha; HNF-1A; HNF1; HNF1 homeobox A; HNF1A; HNF4A; IDDM20; interferon production regulator factor; LF-B1, hepatic nuclear factor (HNF1), albumin proximal factor; LFB1; Liver-specific transcription factor LF-B1; MODY3; TCF-1; TCF1; Transcription factor 1; transcription factor 1, hepatic; truncated hepatocyte nuclear factor 1 alpha Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 81 SOURCE: Rabbit