CST, 54576S, LSD2 (E1R6O) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying LSD2. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, LSD2 (E1R6O) Rabbit Monoclonal Antibody (CST #54576) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity LSD2 (E1R6O) Rabbit Monoclonal Antibody recognizes endogenous levels of total LSD2 protein. This antibody does not cross-react with LSD1 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu467 of human LSD2 protein. Background Lysine-specific demethylase 2 (LSD2; also known as AOF1) is a nuclear amine oxidase homolog that acts as a histone demethylase and transcription cofactor protein (1,2). LSD2 functions as a co-repressor protein by demethylating mono-methyl and di-methyl histone H3 Lys4, two marks associated with actively transcribed genes (1,2). LSD2-mediated demethylation of histone H3 Lys4 is required for establishing proper DNA methylation imprints during oogenesis (3). In addition, LSD2 appears to be overexpressed in malignant breast cancers, where it contributes to DNA methylation and repression of multiple tumor suppressor genes (4,5). Furthermore, LSD2 also contains E3 ubiquitin ligase activity that targets O-GlcNac transferase (OGT) for proteosomal degradation (6). A549 lung cancer cell growth is dependent on this E3 ubiquitin ligase activity, suggesting that this function of LSD2 is also important for proper gene regulation (6). Alternate Names amine oxidase (flavin containing) domain 1; amine oxidase, flavin containing 1; AOF1; bA204B7.3; C6orf193; dJ298J15.2; DKFZp686I0412; Flavin-containing amine oxidase domain-containing protein 1; FLJ33898; FLJ34109; FLJ43328; KDM1B; LSD2; lysine (K)-specific demethylase 1B; lysine demethylase 1B; Lysine-specific histone demethylase 1B; Lysine-specific histone demethylase 2 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 92 Source/Isotype: Rabbit IgG