CST, 54618S, Cytochrome b (E7F8Z) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying MT-CYB/Cytochrome B. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Cytochrome b (E7F8Z) Rabbit Monoclonal Antibody (CST #54618) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Cytochrome b (E7F8Z) Rabbit Monoclonal Antibody recognizes endogenous levels of total cytochrome b protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro208 of human cytochrome b protein. Background Cytochrome b, encoded by the gene , is a subunit of ubiquinol-cytochrome c reductase, also known as cytochrome bc1, or complex III, of the mitochondrial respiratory (electron transport) chain (1). This protein complex mediates electron transfer from ubiquinol to cytochrome c, contributing to the generation of a proton gradient that powers ATP synthesis (2). Cytochrome b is an integral membrane protein and notable for being the only protein within complex III that is encoded by the mitochondrial genome. Genetic sequence analyses have identified mutations in  that are linked to a variety of diseases and pathologies (3,4). Natural variations in the sequence of  have also proven highly useful as a tool to determine phylogenetic relationships between organisms (5). Alternate Names COB; Complex III subunit 3; Complex III subunit III; CYB; CYTB; Cytochrome b; cytochrome b (mitochondrion); Cytochrome b-c1 complex subunit 3; mitochondrially encoded cytochrome b; MT-CYB; MTCYB; Ubiquinol-cytochrome-c reductase complex cytochrome b subunit Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 26 Source/Isotype: Rabbit IgG