CST, 54645S, Bafilomycin A1

Chemical Modulators for studying in the research area. Product Usage Information Bafilomycin A1 is supplied as a lyophilized powder. For a 1 mM stock, reconstitute the 100 μg in 160.56 μl DMSO. Working concentrations and length of treatment can vary depending on the desired effect, but it is typically used at 10 nM-1 μM for up to 18 hr. Storage Store lyophilized or in solution at -20ºC, desiccated. Protect from light. In lyophilized form, the chemical is stable for 24 months. Once in solution, use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles. Background Isolated from s, bafilomycin A1 is a macrolide antibiotic that reversibly inhibits late phase autophagy (1,2). By inhibiting H -ATPase, bafilomycin A1 prevents re-acidification of synaptic vesicles following exocytosis and thereby blocks fusion of autophagosomes and lysosomes (2-5). Research studies demonstrate that bafilomycin A1 can also inhibit cell proliferation (4). Alternate Names autophagosome; autophagy; baf; inhibitor; lysosome