CST, 54653S, Androgen Receptor Antibody (Carboxy-terminal Antigen)

Polyclonal Antibody for studying Androgen Receptor. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, Androgen Receptor Antibody (Carboxy-terminal Antigen) (CST #54653) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Androgen Receptor Antibody (Carboxy-terminal Antigen) recognizes endogenous levels of total Androgen Receptor protein. This antibody does not detect AR-V7 isoform. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val662 of human Androgen Receptor protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Androgen receptor (AR), a zinc finger transcription factor belonging to the nuclear receptor superfamily, is activated by phosphorylation and dimerization upon ligand binding (1). This promotes nuclear localization and binding of AR to androgen response elements in androgen target genes. Research studies have shown that AR plays a crucial role in several stages of male development and the progression of prostate cancer (2,3). Alternate Names AIS; ANDR; Androgen receptor; AR; AR8; DHTR; Dihydrotestosterone receptor; HUMARA; HYSP1; KD; NR3C4; Nuclear receptor subfamily 3 group C member 4; SBMA; SMAX1; TFM Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 110 SOURCE: Rabbit