CST, 54670T, MPP5/PALS1 (OTI5D10) Mouse Monoclonal Antibody

Monoclonal Antibody for studying MPP5/ PALS1. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, MPP5/PALS1 (OTI5D10) Mouse Monoclonal Antibody (CST #54670) is ready to ship. Product Usage Information Western Blotting: 1:1000 - 1:2000 Storage Supplied in PBS (pH 7.3), 1% BSA, 0.02% sodium azide, and 50% glycerol. Stable for 12 months when stored at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity MPP5/PALS1 (OTI5D10) Mouse Monoclonal Antibody recognizes endogenous levels of MPP5/PALS1 protein. Species Reactivity: Human, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with the full-length human recombinant MPP5/PALS1 protein. Background MPP5 (Membrane Palmitoylated Protein 5)/PALS1 (Protein-Associated with Lin7) belongs to the MAGUK (membrane-associated guanylate kinase) family whose members typically function as molecular scaffolds in the assembly of multiprotein complexes. MAGUK family members contain PSD-95-Discs Large-zona occludens-1 (PDZ), Src homology 3 (SH3), and guanylate kinase (GuK) domains (1). In addition, MPP5/PALS1 contains two L27 domains, L27C and L27N, that bind to a multi-PDZ domain protein called Pals1-associated tight junction (PATJ) (1,2). MPP5/PALS1 is essential for cellular and tissue polarity, epithelial cell adhesion, and the formation of tight junctions (2,3). This scaffolding protein is involved in the development of ocular tissue and localizes in the Golgi of the retinal cells (3). Studies have suggested that disruption of MPP5/PALS1 by SARS-CoV small envelope E protein can lead to deterioration of the lung epithelium in infected individuals (4). Alternate Names FLJ12615; MAGUK p55 subfamily member 5; membrane palmitoylated protein 5; membrane protein, palmitoylated 5; membrane protein, palmitoylated 5 (MAGUK p55 subfamily member 5); MPP5; PALS1; protein associated with lin seven 1; protein associated with Lin-7 1; Protein PALS1; stardust Specification REACTIVITY: H R SENSITIVITY: Endogenous MW (kDa): 77 Source/Isotype: Mouse IgG1