CST, 5469S, TTK (D15B7) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying TTK. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, TTK (D15B7) Rabbit Monoclonal Antibody (CST #5469) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity TTK (D15B7) Rabbit Monoclonal Antibody detects endogenous levels of total TTK protein. A background band of unknown origin is detected at 70 kDa. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to a region surrounding Ala560 of human TTK protein. Background TTK (Mps1, PYT) is a cell cycle regulated dual specificity kinase present in rapidly proliferating tissues and cell lines (1-3). TTK localizes to kinetochores and centromeres and is an essential component of the mitotic spindle checkpoint as well as centrosome duplication (4-6). The mitotic checkpoint inhibits entry into anaphase until all chromosomes are attached to the spindle; inhibition of this process leads to genomic instability and tumorigenesis. Phosphorylation of the BLM helicase at Ser144 by TTK maintains chromosome stability during mitosis (7). Small molecule inhibitors of TTK can block the spindle checkpoint response, thereby making TTK a potential therapeutic target (8,9). TTK also participates in the DNA damage response by directly phosphorylating and activating the cell cycle checkpoint kinase Chk2 at Thr68. Two targets phosphorylated by Chk2 are the cell cycle phosphatase cdc25 and the transcription factor p53. Inactivation of cdc25 phosphatase results in the accumulation of inactive cyclin B and cell cycle arrest following DNA damage. Phosphorylation of p53 by active Chk2 stabilizes the transcription factor and promotes cell cycle arrest and apoptosis in response to DNA damage (10). Alternate Names cancer/testis antigen 96; CT96; Dual specificity protein kinase TTK; ESK; FLJ38280; monopolar spindle 1 kinase; monopolar spindle 1-like 1; MPH1; MPS1; MPS1L1; phosphotyrosine picked threonine kinase; Phosphotyrosine picked threonine-protein kinase; PYT; TTK; TTK protein kinase Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 95 Source/Isotype: Rabbit IgG