CST, 5471T, Livin (D61D1) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Livin. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, Livin (D61D1) Rabbit Monoclonal Antibody (CST #5471) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 Immunofluorescence (Immunocytochemistry): 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity Livin (D61D1) Rabbit Monoclonal Antibody detects endogenous levels of total livin protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala195 of human livin protein. Background The inhibitor of apoptosis protein (IAP) family consists of an evolutionarily conserved group of apoptosis inhibitors containing a conserved 70 amino acid BIR (baculovirus inhibitor repeat) domain (1,2). Human members of this family include c-IAP1, c-IAP2, XIAP, survivin, livin, and NAIP. Overexpression of IAP family members, particularly survivin and livin, in cancer cell lines and primary tumors suggests an important role for these proteins in cancer progression (3-5). In general, the IAP proteins function through direct interactions to inhibit the activity of several caspases, including caspase-3, caspase-7, and caspase-9 (5,6). In addition, binding of IAP family members to the mitochondrial protein Smac blocks their interaction with caspase-9, thereby allowing the processing and activation of the caspase (2). Livin (BIRC7/ML-IAP) is a potent anti-apoptotic IAP family member containing a single BIR domain and RING finger motif that is highly expressed in human melanomas and absent in normal tissues (5,7). It is localized in the nucleus and diffusely in the cytoplasm (5). The livin gene encodes two splicing variants, a 298 amino acid isoform (α) and a 280 amino acid form (β), with different biological activity to various apoptotic stimuli (8). In addition to directly inhibiting caspase activity, livin can promote the degradation of the pro-apoptotic protein Smac through the ubiquitin-proteasome pathway (9). Its preferential expression in tumor cells, along with several studies showing that downregulation of livin can promote apoptosis, has led to studies analyzing the use of livin in cancer therapy (10). Alternate Names baculoviral IAP repeat containing 7; baculoviral IAP repeat-containing 7; Baculoviral IAP repeat-containing protein 7; Baculoviral IAP repeat-containing protein 7 30kDa subunit; BIRC7; KIAP; Kidney inhibitor of apoptosis protein; LIVIN; livin inhibitor of apoptosis; livin inhibitor-of-apoptosis; Melanoma inhibitor of apoptosis protein; ML-IAP; MLIAP; p30-Livin; RING finger protein 50; RING-type E3 ubiquitin transferase BIRC7; RNF50; tLivin; Truncated livin Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 34, 36 Source/Isotype: Rabbit IgG