CST, 5474S, Diap2 Antibody

Polyclonal Antibody for studying DIAP2. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, Diap2 Antibody (CST #5474) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Diap2 Antibody recognizes endogenous levels of total diap2 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human diap2 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Formins are a family of large multidomain actin nucleation/polymerization proteins characterized by their catalytic FH2 domains. The mammalian diaphanous-related formin (mDia/diap) subfamily, including mDia1/diap1, mDia2/diap3 and mDia3/diap2, are effectors of Rho family small GTPases. In response to Rho, mDia/diap proteins are involved in the regulation of multiple cell functions including cytoskeletal dynamics, migration, adhesion, polarity and cell shape (reviewed in 1,2). mDia1/diap1 is activated by GTP-bound Rho, leading to Rho-associated kinase (ROCK)-dependent stress fiber formation (3,4). Rho activation of mDia1 has also been shown to regulate serum response factor (SRF)-dependent transcription (5), and has been implicated in human cancer phenotypes such as ras-mediated transformation, metastasis and invasion (reviewed in 6). mDia3/diap2, activated by the Rho family small GTPase cdc42, regulates the attachment of microtubules to the kinetochore during mitosis in mammalian cells (7). Rho-dependent activation of mDia2/diap3 is important in assembly of the contractile ring during cytokinesis (8,9). Alternate Names DIA; DIA2; DIAP2; DIAPH2; diaphanous 2; diaphanous homolog 2; diaphanous homolog 2 (Drosophila); diaphanous related formin 2; Diaphanous-2; Diaphanous-related formin-2; diaphorase-2; DRF2; FLJ11167; mDia3; POF; POF2; POF2A; Protein diaphanous homolog 2 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 130 SOURCE: Rabbit