CST, 5475S, GST-Tag (91G1) Rabbit Monoclonal Antibody (HRP Conjugate)

Monoclonal Antibody for studying . Validated for Western Blotting. Highly specific and rigorously validated in-house, GST-Tag (91G1) Rabbit Monoclonal Antibody (HRP Conjugate) (CST #5475) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 140 mM NaCl, 3 mM KCI, 10 mM sodium phosphate (pH 7.4) dibasic, 2 mM potassium phosphate monobasic, 2 mg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity GST-Tag (91G1) Rabbit Monoclonal Antibody (HRP Conjugate) detects transfected GST fusion proteins. Species Reactivity: All Species Expected Source / Purification Monoclonal antibody is produced by immunizing animals with a GST fusion protein. Background Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties. Glutathione S-transferase (GST) is a widely used fusion partner, since it provides both an easily detectable Tag and a simple purification process with little effect on the biological function of the protein of interest. Numerous vectors containing GST-Tag have been developed for both prokaryotic and eukaryotic systems over the past decade (1-3). Specification REACTIVITY: All SENSITIVITY: Transfected Only Source/Isotype: Rabbit IgG