CST, 5480T, CHAF1A (D77D5) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CHAF1A. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, CHAF1A (D77D5) Rabbit Monoclonal Antibody (CST #5480) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Immunofluorescence (Immunocytochemistry): 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity CHAF1A (D77D5) Rabbit Monoclonal Antibody detects endogenous levels of total CHAF1A protein. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro303 of human CHAF1A protein. Background Chromatin assembly factor 1 (CAF-1) is a histone H3/H4 chaperone complex that functions in de novo assembly of nucleosomes during DNA replication and nucleotide excision repair (1). Nucleosome assembly is a two-step process, involving initial deposition of a histone H3/H4 tetramer onto DNA, followed by the deposition of a pair of histone H2A/H2B dimers (1). CAF-1 interacts with PCNA and localizes to DNA replication and DNA repair foci, where it functions to assemble newly synthesized histone H3/H4 tetramers onto replicating DNA (2-6). Assembly of histone H2A/H2B dimers requires additional assembly factors. The CAF-1 complex consists of three proteins: CHAF1A (p150), CHAF1B (p60) and RBAP48 (p48 or RBBP4). CHAF1A and CHAF1B proteins are specific for the CAF-1 complex, while RBAP48 is a component of multiple chromatin modifying complexes (1). CHAF1A and CHAF1B expression levels correlate with cellular proliferation and both proteins are significantly down-regulated in quiescent cells (7). Alternate Names CAF; CAF-1; CAF-1 subunit A; CAF-1A; CAF-I 150 kDa subunit; CAF-I p150; CAF1; CAF1A; CAF1B; CAF1P150; CHAF1A; Chromatin assembly factor 1 subunit A; chromatin assembly factor 1, subunit A (p150); chromatin assembly factor I (150 kDa); Chromatin assembly factor I p150 subunit; CTB-50L17.7; hp150; MGC71229; P150 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 145 Source/Isotype: Rabbit IgG