CST, 54837S, Fc-gamma RIIB (D8F9C) Rabbit Monoclonal Antibody (Alexa Fluor® 488 Conjugate)

Monoclonal Antibody for studying FcgR2b mouse. Validated for Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, Fc-gamma RIIB (D8F9C) Rabbit Monoclonal Antibody (Alexa Fluor^® 488 Conjugate) (CST #54837) is ready to ship. Product Usage Information Flow Cytometry (Fixed/Permeabilized): 1:50 Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity Fc-gamma RIIB (D8F9C) Rabbit Monoclonal Antibody (Alexa Fluor Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro284 of mouse FcγRIIB protein. Background FcγRIIB (CD32B) is a low affinity, IgG Fc-binding receptor expressed on B cells, monocytes, macrophages, and dendritic cells (DCs) (1-3). It is the inhibitory Fc receptor and signals through an immunoreceptor tyrosine-based inhibitory motif (ITIM) within its carboxy-terminal cytoplasmic tail (2). Binding of immune complexes to FcγRIIB results in tyrosine phosphorylation of the ITIM motif at Tyr292 and recruitment of the phosphatase SHIP, which mediates inhibitory effects on immune cell activation (2,4). In this way, FcγRIIB suppresses the effects of activating Fc-binding receptors (3). For example, mice deficient for FcγRIIB have greater T cell and DC responses following injection of immune complexes (5,6). In addition, FcγRIIB plays a role in B cell affinity maturation (7). Signaling through FcγRIIB in the absence of signaling through the B cell receptor (BCR) is proapoptotic, while signaling through FcγRIIB and the BCR simultaneously attenuates the apoptotic signal and results in selection of B cells with higher antigen affinity (7). Alternate Names AI528646; CD32; F630109E10Rik; Fc gamma receptor IIB; Fc gamma RIIB; Fc receptor, IgG, low affinity IIb; Fc-gamma RII; Fc-gamma-RII; Fc-gamma-RIIB; Fc[g]; Fc[g]RII; Fcg; Fcgamm; FcgammaRIIB; Fcgr; Fcgr2; Fcgr2a; Fcgr2b; FcgRII; Fcr-; Fcr-2; Fcr-3; FcRII; IgG Fc receptor II beta; Low affinity immunoglobulin gamma Fc region receptor II; low affinity immunoglobulin gamma Fc region receptor II-b; Ly-1; Ly-17; Ly-m2; Ly-m20; LyM-; LyM-1; Lymphocyte antigen 17; OTTMUSP00000024374; OTTMUSP00000024375 Specification REACTIVITY: M SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG