CST, 5483S, Phospho-TBK1/NAK (Ser172) (D52C2) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying TBK1 (Ser172) phosphate. Validated for WB,WB,IP,IF,F. Available in 3 sizes. Highly specific and rigorously validated in-house, Phospho-TBK1/NAK (Ser172) (D52C2) Rabbit Monoclonal Antibody (CST #5483) is ready to ship. Product Usage Information Western Blotting: 1:1000 Fluorescent Western: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Immunocytochemistry): 1:50 - 1:100 Flow Cytometry (Fixed/Permeabilized): 1:50 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 54382 . Protocol Available protocols: Western Blotting, Fluorescent Western, Immunoprecipitation, Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity Phospho-TBK1/NAK (Ser172) (D52C2) Rabbit Monoclonal Antibody detects endogenous levels of TBK1 only when phosphorylated at Ser172. This antibody may cross-react with phospho-IKKε. Phospho-TBK1/NAK (Ser172) (D52C2) XP Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is prepared from animals immunized with a synthetic phosphopeptide corresponding to residues surrounding Ser172 of human TBK1. Background TBK1 (TANK-binding kinase 1)/NAK (NF-κB activating kinase) is an IκB kinase (IKK)-activating kinase and can activate IKK through direct phosphorylation (1). TBK1 was identified through association with the TRAF binding protein, TANK, and found to function upstream of NIK and IKK in the activation of NF-κB (2). TBK1 induces IκB degradation and NF-κB activity through IKKβ. TBK1 may mediate IKK and NF-κB activation in response to growth factors that stimulate PKCε activity (1). TBK1 plays a pivotal role in the activation of IRF3 in the innate immune response (3). TBK1 is phosphorylated at Ser172 within its activation loop, which is necessary for its kinase activity including the downstream phosphorylation of IRF3 (4,5). Alternate Names FLJ11330; FTDALS4; IIAE8; NAK; NF-kappa-B-activating kinase; NF-kB-activating kinase; Serine/threonine-protein kinase TBK1; T2K; TANK binding kinase 1; TANK-binding kinase 1; TBK1 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 84 Source/Isotype: Rabbit IgG