CST, 5484S, Phospho-Ezrin (Tyr353) (D66D3) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying ezrin (Tyr354) phosphate. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-Ezrin (Tyr353) (D66D3) Rabbit Monoclonal Antibody (CST #5484) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Phospho-Ezrin (Tyr353) (D66D3) Rabbit Monoclonal Antibody recognizes endogenous levels of ezrin protein only when phosphorylated at Tyr353. Based on protein sequences, this antibody is not expected to cross-react with radixin or moesin. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr353 of human ezrin protein. Background The ezrin, radixin, and moesin (ERM) proteins function as linkers between the plasma membrane and the actin cytoskeleton and are involved in cell adhesion, membrane ruffling, and microvilli formation (1). ERM proteins undergo intra or intermolecular interaction between their amino- and carboxy-terminal domains, existing as inactive cytosolic monomers or dimers (2). Phosphorylation at a carboxy-terminal threonine residue (Thr567 of ezrin, Thr564 of radixin, Thr558 of moesin) disrupts the amino- and carboxy-terminal association and may play a key role in regulating ERM protein conformation and function (3,4). Phosphorylation at Thr567 of ezrin is required for cytoskeletal rearrangements and oncogene-induced transformation (5). Ezrin is also phosphorylated at tyrosine residues upon growth factor stimulation. Phosphorylation of Tyr353 of ezrin transmits a survival signal during epithelial differentiation (6). Alternate Names CVIL; CVL; Cytovillin; cytovillin 2; DKFZp762H157; epididymis secretory protein Li 105; EZR; EZRI; Ezrin; FLJ26216; HEL-S-105; MGC1584; p81; VIL2; villin 2 (ezrin); Villin-2 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 80 Source/Isotype: Rabbit IgG