CST, 54851T, NG2/CSPG4 (F8X3M) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying NG2 mouse. Validated for WB,IP,IF,IF,F,F. Available in 2 sizes. Highly specific and rigorously validated in-house, NG2/CSPG4 (F8X3M) Rabbit Monoclonal Antibody (CST #54851) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Frozen): 1:400 - 1:1600 Immunofluorescence (Immunocytochemistry): 1:50 - 1:200 Flow Cytometry (Fixed/Permeabilized): 1:50 - 1:200 Flow Cytometry (Live): 1:50 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized), Flow Cytometry (Live) Specificity / Sensitivity NG2/CSPG4 (F8X3M) Rabbit Monoclonal Antibody recognizes endogenous levels of total NG2/CSPG4 protein. Nonspecific staining of fixed frozen mouse kidney tubules may be observed by immunofluorescence. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a recombinant protein specific to the central region of mouse NG2/CSPG4 protein. Background The chondroitin sulfate proteoglycan NG2 is a type I membrane protein expressed by subpopulations of glia, including oligodendroglial precursor cells and a variety of tumor cells. Normal precursor cells and malignant tumor cells migrate and proliferate, but there is evidence that cells may not be able to engage in both activities at the same time. However, NG2 is involved in promoting both proliferation and motility (1). The extracellular domain of NG2 sequesters growth factors and binds to both growth factor receptors and extracellular matrix ligands such as fibronectin, collagens, and laminin. The cytoplasmic domain is involved in activating Rac, Cdc42, and p130 Cas (2). PKCα phosphorylates NG2 at Thr2256, triggering the redistribution of NG2 from apical microprocesses to lamellipodia, accompanied by enhanced cell motility (3). ERK phosphorylates NG2 at Thr2314, stimulating cell proliferation (4). Alternate Names 4732461B14Rik; An2; AN2 proteoglycan; AN2/NG2 proteoglycan; Chondroitin sulfate proteoglycan 4; Chondroitin sulfate proteoglycan NG2; Csp; Cspg4; Cspg4a; Kiaa4232; nerve/glial antigen 2; Ng2; Proteoglycan AN2 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 250, 450 Source/Isotype: Rabbit IgG