CST, 5486S, Diap1 Antibody

Polyclonal Antibody for studying DIAP1. Validated for Western Blotting. Highly specific and rigorously validated in-house, Diap1 Antibody (CST #5486) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Diap1 Antibody recognizes endogenous levels of total Diap1 protein. Species Reactivity: Human, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human Diap1 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Formins are a family of large multidomain actin nucleation/polymerization proteins characterized by their catalytic FH2 domains. The mammalian diaphanous-related formin (mDia/diap) subfamily, including mDia1/diap1, mDia2/diap3 and mDia3/diap2, are effectors of Rho family small GTPases. In response to Rho, mDia/diap proteins are involved in the regulation of multiple cell functions including cytoskeletal dynamics, migration, adhesion, polarity and cell shape (reviewed in 1,2). mDia1/diap1 is activated by GTP-bound Rho, leading to Rho-associated kinase (ROCK)-dependent stress fiber formation (3,4). Rho activation of mDia1 has also been shown to regulate serum response factor (SRF)-dependent transcription (5), and has been implicated in human cancer phenotypes such as ras-mediated transformation, metastasis and invasion (reviewed in 6). mDia3/diap2, activated by the Rho family small GTPase cdc42, regulates the attachment of microtubules to the kinetochore during mitosis in mammalian cells (7). Rho-dependent activation of mDia2/diap3 is important in assembly of the contractile ring during cytokinesis (8,9). Alternate Names DFNA1; DIA1; DIAP1; DIAPH1; diaphanous homolog 1 (Drosophila); diaphanous related formin 1; Diaphanous-1; diaphanous-related formin 1; Diaphanous-related formin-1; DRF1; FLJ25265; hDIA1; LFHL1; Protein diaphanous homolog 1; SCBMS Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 150 SOURCE: Rabbit