CST, 54922T, MYO10 (F3V7U) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying MYO10. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, MYO10 (F3V7U) Rabbit Monoclonal Antibody (CST #54922) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Immunofluorescence (Immunocytochemistry): 1:50 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity MYO10 (F3V7U) Rabbit Monoclonal Antibody recognizes endogenous levels of total MYO10 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the central region of human MYO10 protein. Background Myosin family motor proteins drive ATP-dependent actin-based motility in eukaryotic cells and contain a conserved amino-terminal motor domain (1). Unconventional myosin-X (MYO10) binds actin filaments and localizes to filopodia tips. MYO10 regulates cell migration, metastasis, and neuritogenesis, and its expression is upregulated in mouse and human tumors (2). MYO10 was shown to drive tumor progression by inducing genome instability through cGAS/STING inflammatory response (3). Regulation of MYO10 phosphorylation and protein levels in mitosis is critical for spindle formation, genome stability, and sensitivity of cancer cells to Taxol (4). Alternate Names FLJ10639; FLJ21066; FLJ22268; FLJ43256; KIAA0799; MGC131988; MYO10; myosin X; Myosin-X; Unconventional myosin-10; Unconventional myosin-X; unconventionnal myosin-X Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 237 Source/Isotype: Rabbit IgG