CST, 54934T, PTN (F7T3F) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PTN. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, PTN (F7T3F) Rabbit Monoclonal Antibody (CST #54934) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Immunocytochemistry): 1:1600 - 1:6400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity PTN (F7T3F) Rabbit Monoclonal Antibody recognizes endogenous levels of total PTN protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human PTN protein. Background Pleiotrophin (PTN) is a heparin-binding, developmentally regulated cytokine that is independently identified by several groups; it is also known as heparin-binding brain mitogen (HBBM), heparin-binding growth factor-8 (HBGF-8), heparin-binding neurotrophic factor (HBNF), heparin-affinity regulatory peptide (HARP), heparin-binding growth-associated molecule (HB-GAM), and osteoblast-specific factor 1 (OSF-1) (1-6). PTN is highly upregulated during embryonic development and early cell differentiation. In adulthood, PTN expression decreases in most tissues except for the bone and the nervous system. It interacts with numerous cell surface proteoglycan and non-proteoglycan receptors, including protein tyrosine phosphatase receptor type Z (PTPRZ), syndecans, nucleolin, neuropilin-1, ALK, and integrin αVβ3 and αMβ2 (reviewed in 7-9). PTN has been associated with biological events that involve cellular proliferation, differentiation, and inflammation, including tissue regeneration, bone repair, hypoxia, tumor growth, and angiogenesis. Alternate Names HARP; HB-GAM; HBBM; HBGF-8; HBGF8; HBNF; HBNF-1; HBNF1; heparin affin regulatory protein; heparin binding growth factor 8; Heparin-binding brain mitogen; Heparin-binding growth factor 8; Heparin-binding growth-associated molecule; heparin-binding neurite outgrowth promoting factor; Heparin-binding neurite outgrowth-promoting factor; Heparin-binding neurite outgrowth-promoting factor 1; NEGF1; neurite growth-promoting factor 1; OSF-1; Osteoblast-specific factor 1; Pleiotrophin; pleiotrophin (heparin binding growth factor 8, neurite growth-promoting factor 1); PTN Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 18 Source/Isotype: Rabbit IgG