CST, 55003T, Arginase-2 (D9J1N) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying ARG2. Validated for WB,IP,IHC,F. Available in 2 sizes. Highly specific and rigorously validated in-house, Arginase-2 (D9J1N) Rabbit Monoclonal Antibody (CST #55003) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Immunohistochemistry (Paraffin): 1:2000 Flow Cytometry (Fixed/Permeabilized): 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 77668 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity Arginase-2 (D9J1N) Rabbit Monoclonal Antibody recognizes endogenous levels of total arginase-2 protein. This antibody does not cross-react with arginase-1 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human arginase-2 protein. Background Arginase-2 is a mitochondrial enzyme that catalyzes the hydrolysis of L-arginine to L-ornithine and urea (1). Research studies have shown that in acute myeloid leukemia (AML) patients, arginase-2 is released from AML blasts to the plasma, leading to the suppression of T-cell proliferation (2). It was also shown that arginase-2 is required for the immunosuppressive properties of neonatal CD71(+) erythroid cells, which inhibits neonatal host defense against infection (3). In addition, the expression of arginase-2 in dendritic cells is repressed by microRNA-155 during maturation (4). This repression is essential for T-cell activation and response (4). Alternate Names ARG2; ARGI2; arginase 2; Arginase II; Arginase-2, mitochondrial; arginase, type II; kidney arginase; Kidney-type arginase; L-arginine amidinohydrolase; L-arginine ureahydrolase; Non-hepatic arginase; nonhepatic arginase; Type II arginase Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 40 Source/Isotype: Rabbit IgG