CST, 55011T, CD93/C1qR (E5H9B) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CD93. Validated for WB,IP,IHC,F. Available in 2 sizes. Highly specific and rigorously validated in-house, CD93/C1qR (E5H9B) Rabbit Monoclonal Antibody (CST #55011) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunohistochemistry (Paraffin): 1:800 - 1:3200 Flow Cytometry (Live): 1:400 - 1:1600 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 55570 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Flow Cytometry (Live) Specificity / Sensitivity CD93/C1qR (E5H9B) Rabbit Monoclonal Antibody recognizes endogenous levels of total CD93/C1qR protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the extracellular domain of human CD93/C1qR protein. Background CD93, also known as complement component C1q receptor (C1qR), is a type-I transmembrane glycoprotein belonging to the CLEC14 family of surface antigens, which share a similar ectodomain architecture highlighted by the presence of a C-type lectin domain and multiple EGF-like domains. CD93/C1qR expression is primarily restricted to endothelial cells associated with normal and tumor-associated vasculature as well as immune cells of the myeloid lineage, including monocytes and neutrophils (1). The extracellular domain of CD93/C1qR is rich in O-linked glycosylation modifications, which stabilizes its surface expression by protecting it from proteolytic cleavage (2,3). CD93/C1qR regulates various aspects of endothelial cell biology, including proliferation, migration, and adhesion (4). Indeed, research studies have demonstrated that surface-expressed CD93/C1qR binds to an endothelial-specific extracellular matrix ligand, multimerin-2, and disrupting the interaction compromises angiogenesis (5). Furthermore, CD93 expression is upregulated in tumor vasculature and correlates with poor survival (6-8). Alternate Names C1q receptor 1; C1q/MBL/SPA receptor; C1qR; C1qR(p); C1QR1; C1qRp; CD93; CD93 antigen; CD93 molecule; CDw93; Complement component 1 q subcomponent receptor 1; complement component 1, q subcomponent, receptor 1; Complement component C1q receptor; dJ737E23.1; ECSM3; Matrix-remodeling-associated protein 4; matrix-remodelling associated 4; MXRA4 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 120, 82 Source/Isotype: Rabbit IgG