CST, 55041P, Phospho-SMAD2 (Ser465/Ser467) (E8F3R) Rabbit Monoclonal Antibody (Biotinylated)

Monoclonal Antibody for studying Smad2 (Ser465/Ser467) phosphate. Validated for Western Blotting,Peptide ELISA (DELFIA). Highly specific and rigorously validated in-house, Phospho-SMAD2 (Ser465/Ser467) (E8F3R) Rabbit Monoclonal Antibody (Biotinylated) (CST #55041) is ready to ship. Product Usage Information Biotinylated antibodies are ideal for immunoassay technologies and high-throughput ELISA platforms that require antibody pairs where both antibodies are from the same host. Platforms utilizing biotinylated antibodies include, but are not limited to, MSD, xMAP, Quanterix Simoa, AlphaLISA, AlphaScreen, HTRF, LANCE, and TR-FRET. Optimal dilutions/working concentrations should be determined by the end user. Please contact us if you require the antibody clone biotinylated at a different concentration, a carrier-free formulation, or a more customized packaging solution Storage Supplied in 140 mM NaCl, 3 mM KCl, 10 mM sodium phosphate (pH 7.4) dibasic, 2 mM potassium phosphate monobasic, 2 mg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Phospho-SMAD2 (Ser465/Ser467) (E8F3R) Rabbit Monoclonal Antibody (Biotinylated) recognizes endogenous levels of Smad2 protein when phosphorylated at Ser465 and Ser467. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser465/Ser467 of human Smad2 protein. Background Members of the SMAD family of signal transduction molecules are components of a critical intracellular pathway that transmit TGF-β signals from the cell surface into the nucleus. Three distinct classes of SMADs have been defined: the receptor-regulated SMADs (R-SMADs), which include SMAD1, 2, 3, 5, and 9; the common-mediator SMAD (co-SMAD), SMAD4; and the antagonistic or inhibitory SMADs (I-SMADs), SMAD6 and 7 (1-5). Activated type I receptors associate with specific R-SMADs and phosphorylate them on a conserved carboxy-terminal SSXS motif. The phosphorylated R-SMADs dissociate from the receptor and form a heteromeric complex with SMAD4, initiating translocation of the heteromeric SMAD complex to the nucleus. Once in the nucleus, SMADs recruit a variety of DNA binding proteins that function to regulate transcriptional activity (6-8). Alternate Names hMAD-2; hSMAD2; JV18; JV18-1; MAD homolog 2; Mad protein homolog; Mad-related protein 2; MAD, mothers against decapentaplegic homolog 2; MADH2; MADR2; MGC22139; MGC34440; mother against DPP homolog 2; Mothers against decapentaplegic homolog 2; Mothers against DPP homolog 2; Sma- and Mad-related protein 2; SMAD 2; SMAD family member 2; SMAD, mothers against DPP homolog 2; SMAD2 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 60 Source/Isotype: Rabbit IgG