CST, 55051S, CD63 (D4I1X) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CD63. Validated for Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, CD63 (D4I1X) Rabbit Monoclonal Antibody (CST #55051) is ready to ship. Product Usage Information Immunohistochemistry (Paraffin): 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 43890 . Protocol Available protocols: Immunohistochemistry (Paraffin) Specificity / Sensitivity CD63 (D4I1X) Rabbit Monoclonal Antibody recognizes endogenous levels of total CD63 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant CD63 protein. Background CD63 belongs to the tetraspanin family, which is characterized by four transmembrane domains, one short extracellular domain (ECL1), and one long extracellular domain (ECL2) (1-3). Tetraspanins interact with a variety of cell surface proteins and intracellular signaling molecules in specialized tetraspanin-enriched microdomains (TEMs) where they mediate a range of processes, including adhesion, motility, membrane organization, and signal transduction (3). CD63, like other tetraspanins, is enriched in exosomes (4). It is also a component of Weibel-Palade bodies found in endothelial cells (5). Research studies demonstrate several functions of CD63 in different cell types, including roles in mast cell degranulation, VEGF signaling in endothelial cells, recruitment of leukocytes to endothelial cells, and endosomal sorting during melanogenesis (6-9). Alternate Names CD63; CD63 antigen; CD63 antigen (melanoma 1 antigen); CD63 molecule; Granulophysin; LAMP-3; Lysosomal-associated membrane protein 3; lysosome-associated membrane glycoprotein 3; ME491; melanoma 1 antigen; Melanoma-associated antigen ME491; melanoma-associated antigen MLA1; MLA1; Ocular melanoma-associated antigen; OMA81H; Tetraspanin-30; Tspan-30; TSPAN30 Specification REACTIVITY: H SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG