CST, 55091S, HSP20 (E6O7A) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying HSP20/HSPB6. Validated for Western Blotting,Immunohistochemistry (Paraffin),Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, HSP20 (E6O7A) Rabbit Monoclonal Antibody (CST #55091) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:50 - 1:200 Immunofluorescence (Immunocytochemistry): 1:200 - 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity HSP20 (E6O7A) Rabbit Monoclonal Antibody recognizes endogenous levels of total HSP20 protein. Species Reactivity: Human, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro25 of human HSP20 protein. Background The small heat shock protein 20 (HSP20/HSPB6) is a ubiquitously expressed molecular chaperone, with higher levels found in skeletal, cardiac, and smooth muscle (1). HSP20 plays a cardioprotective role in response to injury through cardiac β-adrenergic signaling, which induces increased HSP20 protein expression and phosphorylation at Ser16 by PKA (2-4). Phosphorylation of HSP20 at Ser16 protects cells from undergoing apoptosis (5) and mediates HSP20 interaction with the cytoskeleton (6). Constitutive phosphorylation of HSP20 at Ser16 in mice leads to fibrosis and heart failure through interleukin-6 (IL-6) and STAT3 signaling (7). Alternate Names epididymis luminal protein 55; epididymis secretory sperm binding protein; FLJ32389; Heat shock 20 kDa-like protein p20; Heat shock protein beta-6; heat shock protein family B (small) member 6; heat shock protein family B (small) member B6; heat shock protein, alpha-crystallin-related, B6; HEL55; Hsp20; HSPB6; PPP1R91; protein phosphatase 1, regulatory subunit 91 Specification REACTIVITY: H R SENSITIVITY: Endogenous MW (kDa): 17 Source/Isotype: Rabbit IgG