CST, 55104S, FKBP1A/FKBP12 Antibody

Polyclonal Antibody for studying FKBP1A/FKBP12. Validated for Western Blotting,Simple Western™. Highly specific and rigorously validated in-house, FKBP1A/FKBP12 Antibody (CST #55104) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity FKBP1A/FKBP12 Antibody recognizes endogenous levels of total FKBP1A/FKBP12 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Phe49 of human FKBP1A/FKBP12 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background FKBP12 (FKBP1A) is a prototypical member of the FKBP (FK506 binding protein) family of immunophilins, so named because of the ability of FKBP12 to bind to the immunosuppressive drug FK506 (tacrolimus) (1). The protein is the smallest member in the family and contains only one peptidylprolyl isomerase (PPIase) core domain (FK domain) responsible for its PPIase activity and its binding to FK506 and other compounds (e.g., rapamycin). When bound to FK506 or rapamycin, the protein:drug complex further binds and inhibits two important signaling molecules: calcineurin, a key enzyme in T cell activation, and the metabolic enzyme mTOR. The inhibition of these pathways has been functionally linked to immunosuppression (1,2). Through its binding properties, FKBP12 also plays regulatory roles in other pathways. For example, the ryanodine receptor (RyR), a type of Ca release channel, exhibits leakiness in the absence of FKBP12 binding, leading to reduced muscle contractility (3). FKBP12 can also bind TGFBR1 and prevent ligand independent activation (4). The protein also mediates MDM2 degradation by binding and disrupting MDM2/MDM4 association, thereby inducing MDM2 self-ubiquitination and enhancing the sensitivity of cells to chemotherapy induced cellular apoptosis (5). Alternate Names 12 kDa FK506-binding protein; 12 kDa FKBP; Calstabin-1; FK506 binding protein 1A, 12kDa; FK506 binding protein12; FK506-binding protein 1; FK506-binding protein 12; FK506-binding protein 1A; FK506-binding protein 1A (12kD); FK506-binding protein, T-cell, 12-kD; FKB1A; FKBP prolyl isomerase 1A; FKBP-12; FKBP-1A; FKBP1; FKBP12; FKBP12-Exip3; FKBP12C; FKBP1A; Immunophilin FKBP12; Peptidyl-prolyl cis-trans isomerase FKBP1A; PKC12; PKCI2; PPIASE; PPIase FKBP1A; protein kinase C inhibitor 2; Rotamase Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 12 SOURCE: Rabbit