CST, 5512S, Plexin A3 (D2G12) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PLXNA3. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Plexin A3 (D2G12) Rabbit Monoclonal Antibody (CST #5512) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Plexin A3 (D2G12) Rabbit Monoclonal Antibody detects endogenous levels of total Plexin A3 protein. This antibody may cross-react with other family members. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide surrounding Val440 of human Plexin A3. Background Class 3 secreted semaphorin (Sema3A) is a chemorepellent that acts upon a wide variety of axons. As such, it induces a dramatic redistribution and depolymerization of actin filaments that results in growth cone collapse. Plexins are single membrane-spanning signaling proteins encompassing Plexin A1, A2, A3, and A4. Plexins form a complex with neuropilin-1 and -2 and the cell adhesion protein L1 to form a functional semaphorin receptor (1,2). The GTPase Rnd1 binds to the cytoplasmic domain of Plexin A1 to trigger cytoskeletal collapse. In contrast, the GTPase RhoD blocks Rnd1-mediated Plexin A1 activation and repulsion of sympathetic axons by Sema3A (3). Both Plexin A3 and A4 mediate the responses to class 3 semaphorins in sensory and sympathetic neurons. In particular, Plexin A4 is responsible for signaling of Sema 3A via neuropilin-1, while Plexin A3 is responsible for signaling of Sema 3F via neuropilin-2 (4). Alternate Names 6.3; HSSEXGENE; plexin 4; plexin A3; Plexin-4; Plexin-A3; PLXA3; PLXN3; PLXN4; PLXNA3; Semaphorin receptor SEX; SEX; Sex chromosome X transmembrane protein of HGF receptor family 3; XAP-6 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 207 Source/Isotype: Rabbit IgG