CST, 55159T, COX1/MT-CO1 (E2I2R) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying COX1/MT-CO1. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, COX1/MT-CO1 (E2I2R) Rabbit Monoclonal Antibody (CST #55159) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity COX1/MT-CO1 (E2I2R) Rabbit Monoclonal Antibody recognizes endogenous levels of total COX1/MT-CO1 protein. This antibody does not cross-react with COX2/MT-CO2 protein or COX3/MT-CO3 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human COX1/MT-CO1 protein. Background The mitochondrial electron transport chain comprises multiple protein complexes including cytochrome c oxidase. Cytochrome c oxidase catalyzes the reduction of oxygen to water. This process is coupled with pumping protons from the mitochondrial matrix into mitochondrial intermembrane space, contributing to the proton gradient used for ATP synthesis (1). Cytochrome c oxidase consists of 3 mitochondrial DNA-encoded subunits (COX1/MT-CO1, COX2/MT-CO2, and COX3/MT-CO3) and multiple nuclear DNA-encoded subunits (2). Research studies show that the mRNAs of the mitochondrially encoded oxidative phosphorylation subunits, including COX1/MT-CO1, decline significantly during aging (3). Alternate Names COI; COX1; COXI; cytochrome C oxidase; Cytochrome c oxidase polypeptide I; Cytochrome c oxidase subunit 1; cytochrome c oxidase subunit I; mitochondrially encoded cytochrome c oxidase I; MT-CO1; MT-COI; MTCO1 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 32 Source/Isotype: Rabbit IgG