CST, 5519T, JIP4/SPAG9 (D72F4) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying JIP4. Validated for WB,IP,IHC,IF,F. Available in 2 sizes. Highly specific and rigorously validated in-house, JIP4/SPAG9 (D72F4) Rabbit Monoclonal Antibody (CST #5519) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunohistochemistry (Paraffin): 1:50 Immunofluorescence (Immunocytochemistry): 1:50 Flow Cytometry (Fixed/Permeabilized): 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 49354 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity JIP4/SPAG9 (D72F4) Rabbit Monoclonal Antibody recognizes endogenous levels of total JIP4 protein. This antibody will detect known splice variants of JIP4 (SPAG9, JLP) but is not predicted to cross-react with other JIP family members. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to a region surrounding Leu237 of human JIP4 protein. Background JNK-Interacting Proteins (JIPs), as their name implies, coordinate c-Jun N-terminal Kinase (JNK) signaling by acting as scaffolds for components of the JNK signaling cascade (1). JIPs localize and promote JNK activation in response to stress by amalgamating and co-localizing upstream kinases and downstream effectors in the stress-kinase pathway analogous to the mechanism by which AKAPs orchestrate PKA signaling. JIPs bind to an array of MAPKs and other signaling proteins, including the mixed-lineage kinases, MKK7, p38α MAPK, JNK1-3, Max, Myc, NF-κB, LRRK2, and others (1-4). There are four known JIPs, JIP1-4, of which JIP1 and JIP2 share extensive sequence homology and domain structure. JIP1 and JIP2 are mainly expressed in neurons, testis and in β pancreatic cells, where they have been implicated in cellular responses to metabolic stress, the development of diabetes, and post-traumatic brain damage (5-7). Although architecturally distinct from JIP1 and JIP2, JIP3 and JIP4 share some overlapping functions and are more broadly expressed. JIP4, encoded by the SPAG9 (sperm-associated antigen-9) gene, is a homooligomer that binds to and coordinates the activation of numerous components of the stress-activated kinase cascade including MEK4, MEKK3, p38α MAPK, and JNK1-3 (1,3). However, unlike the other JIP members, JIP4 does not appear to activate JNK directly, instead favoring stimulation of p38 MAPK signaling events in response to cellular stress (3,8). In addition to mediating stress responses, JIP4 (or its splice variant, JLP) has also been shown to interact with ARF6 and PIKfyve, thus regulating microtubule-based endosomal trafficking (9,10). There are extensive reports indicating that JIP4 is phosphorylated in response to stress (UV damage etc.) but it is unclear what effect, if any, this has on its function, localization, or binding properties (11-14). Alternate Names c-Jun NH2-terminal kinase-associated leucine zipper protein; C-Jun-amino-terminal kinase-interacting protein 4; Cancer/testis antigen 89; CT89; FLJ13450; FLJ14006; FLJ26141; FLJ34602; HLC-4 protein; HLC-6; HLC4; HLC6; HSS; Human lung cancer oncogene 6 protein; JIP-4; JIP4; JLP; JNK interacting protein; JNK-associated leucine-zipper protein; JNK-interacting protein 4; JNK/SAPK-associated protein; KIAA0516; lung cancer oncogene 4; MAPK8IP4; Max-binding protein; MGC117291; MGC14967; MGC74461; Mitogen-activated protein kinase 8-interacting protein 4; PHET; PIG6; proliferation-inducing gene 6; Proliferation-inducing protein 6; Protein highly expressed in testis; SPAG9; sperm associated antigen 9; sperm associated antigen 9 variant 5; sperm specific protein; Sperm surface protein; Sperm-associated antigen 9; Sperm-specific protein; Sunday driver 1; SYD1 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 190 Source/Isotype: Rabbit IgG