CST, 55205S, Hexokinase II (F9Q4T) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Hexokinase 2. Validated for WB,WB,IP,F. Highly specific and rigorously validated in-house, Hexokinase II (F9Q4T) Rabbit Monoclonal Antibody (CST #55205) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunoprecipitation: 1:50 Flow Cytometry (Fixed/Permeabilized): 1:600 - 1:2400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity Hexokinase II (F9Q4T) Rabbit Monoclonal Antibody recognizes endogenous levels of total hexokinase II protein. This antibody does not cross-react with hexokinase I protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human hexokinase II protein. Background Hexokinase catalyzes the conversion of glucose to glucose-6-phosphate, the first step in glycolysis. Four distinct mammalian hexokinase isoforms, designated as hexokinase I, II, III, and IV (glucokinase), have been identified. Hexokinases I, II, and III are associated with the outer mitochondrial membrane and are critical for maintaining an elevated rate of aerobic glycolysis in cancer cells (Warburg Effect) (1) in order to compensate for the increased energy demands associated with rapid cell growth and proliferation (2,3). Alternate Names DKFZp686M1669; hexokinase 2; Hexokinase type II; Hexokinase-2; hexokinase-2, muscle; Hexokinase-B; HK II; HK2; HKII; HXK2; Muscle form hexokinase Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 102 Source/Isotype: Rabbit IgG